Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.

doi: 10.4049/jimmunol.175.9.6177

Figure Lengend Snippet: FIGURE 1. IL-17R expression on human NSCLC lines. Three different human NSCLC lines, Sq-19, A549, and LK-87, were incubated with mouse anti-human IL-17R mAb (solid line) or irrelevant mouse IgG1 (dotted line), followed by PE-conjugated goat anti-mouse IgG1 Ab. The expression of IL-17R on the surface of NSCLC cells was analyzed by flow cytometry.

Article Snippet: Cells (1 106), washed twice with PBS containing 2% FCS and 0.1% NaN3, were incubated with mouse anti-human IL-17R mAb or with irrelevant normal mouse IgG1 (R&D Systems) at 4°C for 60 min. After washing, the cells were incubated with PE-conjugated goat anti-mouse IgG1 Ab (Caltag Laboratories) at 4°C for 30 min.

Techniques: Expressing, Incubation, Cytometry

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.

doi: 10.4049/jimmunol.175.9.6177

Figure Lengend Snippet: FIGURE 9. IL-17 and IL-17R expression and vascularity in human NSCLC tissues. A, Total cellular RNA was extracted from human NSCLC tissues. Five micrograms of total RNA were applied for the synthesis of cDNAs. IL-17 mRNA expression was detected in five of seven NSCLC

Article Snippet: Cells (1 106), washed twice with PBS containing 2% FCS and 0.1% NaN3, were incubated with mouse anti-human IL-17R mAb or with irrelevant normal mouse IgG1 (R&D Systems) at 4°C for 60 min. After washing, the cells were incubated with PE-conjugated goat anti-mouse IgG1 Ab (Caltag Laboratories) at 4°C for 30 min.

Techniques: Expressing

Journal: Science Advances

Article Title: Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments

doi: 10.1126/sciadv.aba6712

Figure Lengend Snippet: ( A ) After 7 days of incubation, a factor of 9.4 greater population of motile E. gracilis cells per droplet was identified in large SADA-sorted droplets (110 pl) than in small SADA-sorted droplets (26 pl). Insets show photos of typical trapped large and small droplets (110 and 26 pl) containing E. gracilis cells. The droplets shown are exactly the same droplets across days. Scale bars, 50 μm. ( B ) After 18 and 12 hours of incubation, 4.7 and 4.9 times higher viability is observed for Jurkat cells and a B5F6 hybridoma clone, respectively, in large SADA-sorted droplets (110 pl) than in small SADA-sorted droplets (26 pl). The incubation time began when the sorting process was finished. The sample size ( n ) for each dataset is available in data file S2. ( C ) Higher antibody production rate for the B5F6 clone in large SADA-sorted droplets (110 pl) than in small SADA-sorted droplets (26 pl). Each dot indicates one measurement taken from a different sample.

Article Snippet: A hybridoma cell line (B5F6, anti-mouse IL-17RB mAb-producing line) has been established as described previously ( ) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (MP Biomedicals), 10% BM Condimed H1 (Roche), and penicillin (100 U/ml) and streptomycin (100 μg/ml; Wako Chemicals) in a cell incubator at 37°C and 5% CO 2 .

Techniques: Incubation